Sentence examples for amplification approaches from inspiring English sources
The other is that aptamers are more easily employed for developing highly sensitive detection methods by using various nucleic acid-based signal amplification approaches.
Recently, enzyme-assisted amplification approaches have provided useful platforms for the telomerase activity detection, however, further improvement in sensitivity is still hindered by the single-step signal amplification.
These signal amplification approaches were demonstrated and show that this system is capable of specific detection of bacteria (Escherichia coli) and proteins (ovalbumin).
Among the signal amplification approaches in highly sensitive aptasensors, the nuclease-based target recycling signal amplification has recently become a research focus because it shows easy design, simple operation, and rapid reaction and can be easily developed for homogenous assay.
Such a DNA nanoassembly endows the genosensor an ultrahigh sensitivity up to 1 aM, which is higher than that of the nanomaterials-based or enzyme mediated amplification approaches by several orders of magnitude.
Whilst there are several very elegant isothermal amplification approaches, multiplexed amplification remains a challenge, requiring careful experimental design and optimisation, from judicious primer design in order to avoid the formation of primer dimers and non-specific amplification, applied temperature as well as the ratio and concentration of primers.
Loop-mediated isothermal amplification (LAMP) is a DNA amplification approach characterized by high sensitivity and specificity.
A key to the success of this signal amplification approach is an appropriate choice of the amplification agent.
Therefore, a dual amplification approach is provided for nucleic acid phosphorylation related researches.
We have developed methods for full-genome sequencing of Zika viruses (ZIKVs) based on a targeted amplification approach.
We combined a three-primer polymerase chain reaction (PCR) amplification approach with HRM using two primer sets.
