Sentence examples for amplification analysis of from inspiring English sources
Standard methods were used for PCR amplification, analysis of DNA fragments and electroporation as directed by Green and Sambrook (2012).
Amplification analysis of the 16S rRNA gene were developed by polymerase chain reactions (PCR), using 27f and 1401r (Lane 1991) as primers at the following conditions: DNA 6.5 µL, Taq Buffer 5 µL; MgCl2 (2.5 nM) 1.5 µL, dNTP's (2.5 nM) 0.4 µL, primers (20 µM) 1 µL, Taq DNA polymerase (Invitrogen) 0.4 U/µL.
N. meningitidis-specific gene amplification analysis of N. meningitidis PCR-positive CSF with the presence of contact-regulated gene A (crgA), IS1106, and 16S rRNA was conducted.
Gene amplification analysis of 1083 solid tumours found that amplification of the EPOR locus was rare with frequencies similar to other non-oncogenes.
Multiple ligation-dependent probe amplification analysis of the NF2 gene demonstrated no additional copy number aberrations in blood or tumours of this patient (data not shown).
Sequencing of the large and structurally complex PKD1 gene is usually the first step; if negative, it is followed by PKD2 sequencing and finally MLPA (multiplex ligation-dependent probe amplification) analysis of both genes to detect larger deletions.
Furthermore, fluorescent hydrolysis probe technology was integrated into iiPCR to eliminate post-amplification analysis of amplicons [ 46].
A gene-amplification analysis of 1,083 solid tumors showed that EpoR-gene amplification was rare; overall, the EpoR-gene frequency was similar to other non-oncogenes [ 47].
When performing simultaneous amplification and analysis of all 12 species some preferential amplification occurred, as not all peaks could be observed.
For each amplification reaction, analysis of the product dissociation curve was performed to exclude the presence of non-specific amplification.
These include amplification and analysis of the resonator output signal43,44 and measurement of the resonator occupation using a superconducting qubit8,45.
