Sentence examples for amounts were determined using from inspiring English sources

Ca2+ and Mg2+ amounts were determined using a conventional colorimetric EDTA titration.

Protein amounts were determined using a bovine serum albumin calibration curve.

Glucose and glucose plus glycogen amounts were determined using a standard curve and were normalized to the amount of protein.

Gene expression amounts were determined using the 2-ΔT method, where ΔT is the count difference of the candidate gene from TF2B.

Protein amounts were determined using the Pierce BCA protein assay (Thermo Fisher Scientific, Rockford, IL, USA) and equal amounts of proteins were resolved by SDS PAGE using 4 12% gels (NuPAGE, Invitrogen Life Technologies).

MET DNA amounts were determined using an Applied Biosystems 7500 Real-Time PCR System Applied Biosystemss, Foster City, CA, USA), and the data were analysed using SDS (Ver. 1.4) software (Applied Biosystems).

Protein amount was determined using the method of Lowry et al. [ 29].

The protein amount was determined using the Bio-Rad protein assay dye (Bio-Rad, Glattbrugg, Switzerland).

The total protein amount was determined using a standard curve for bovine serum albumin (BSA).

Subsequently, total protein amount was determined using BCA protein assay (Thermo Scientific Pierce, Rockford, USA) and 10 μg of each sample were proteolytic digested with trypsin (Promega, Mannheim, Germany).

However, if the respondents were willing to pay less than the minimum offered bid or higher than the maximum offered bid their WTP amount was determined using open-ended questions.