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Protein amounts were assessed using the BCA Protein Assay Kit (Pierce Biotechnology, Rockford, IL), with bovine serum albumin as a standard.
The polycistronic region nad5ex4-atp4-nad5ex5 in MSC3 and rps7 in MSC12 and MSC16 were hypothesized to be likely candidates for their respective MSC phenotypes and their relative amounts were assessed using quantitative (q) PCR.
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Amount of total phenolic contents were assessed using Folin-Ciocalteu reagent procedure [32].
The amount and quality of RNA were assessed using a UV spectrophotometer.
The amount and quality of RNA were assessed using NanoDrop 2000 (Thermo Scientific) at 260/280 with all samples having values between 1.8 and 2.0.
Hunger, fullness, desire to eat, amount of food consumed, and thirst were assessed using a modified Visual Analog Scale (VAS) before and after the breakfast, snack and lunch.
Costs were assessed using the amount paid to providers for a claim or health service, including copayment(s) and/or coinsurance, and were reported on a per-patient per-year basis.
Embryonic phenotypes were then analyzed using the neuronal marker anti-HRP and the relative amounts of Fra, Robo or RoboSD proteins were assessed using anti-HA antibodies.
Differences in the amount of Aβ in control and AD human brain were assessed using two-tailed student t-tests.
Protein concentrations were assessed using the Pierce ™ BCA Protein Assay Kit
Time trends were assessed using birth cohorts.
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