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Samples were normalized to Gapdh and relative quantitative amounts were analyzed using the cycle threshold method (2^-ΔΔCt).
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Specific protein amount was analyzed using the standard procedure of western blot analysis.
The relative amounts of expressed proteins compared to total GAPDH were analyzed using Multi Gauge 3.0 software (Fuji, Tokyo).
With the objective of evaluating significant differences among the different treatment plots for all soil parameters following the application of different amounts of vermicomposts, data were analyzed using one way ANOVA at 0.05 % level of significance (Zar 2009).
The amounts of the proteins were analyzed using ImageJ analysis software version 1.38e (National Institutes of Health, Bethesda, MD) and normalized to their respective controls.
The integrity and amount of purified RNA were analyzed using the RNA 6000 Pico Assay kit (Agilent Technologies, Barcelona, Spain).
When these amounts of extracted fly fat were analyzed using the coupled colorimetric assay kits, no signal at all was observed (Fig. 1F).
Diet choice preferences (amount eaten, cached, and declined) were analyzed using repeated measures ANOVAs across all trials.
Triglyceride levels were normalized to protein amounts in each homogenate, and data were analyzed using a Student's t test.
Changes in the amount of two types of crystals were analyzed using the WAXD integrated intensity.
Experiments were done the indicated amount of times, and the data were analyzed using a Student's paired t-test.
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