Sentence examples for amounts of the appropriate from inspiring English sources

dsDNA and pDNA duplexes were obtained by mixing equimolar amounts of the appropriate oligonucleotides in 20 mM Tris-HCl buffer pH 7.8, 25 mM NaCl, at 93 °C for 2 min with subsequent annealing by slow cooling at room temperature.

Equimolar amounts of the appropriate (3), acrylonitrile, acrylamide or the appropriate N-arylmalemides (7a c) (0.005 mol each) in toluene (30 ml) were heated under reflux for 18 h.

Equal amounts of the appropriate immobilized GST fusion proteins were incubated with normalized whole cell lysates by rocking for 3 h at 4°C.

Similar amounts of the appropriate solvent were added to the control cells.

For reporter assays, Saos-2 p53 Tet-on cells were transfected with 0.5 μg of luciferase reporter and varying amounts of the appropriate expression vector using Lipofectamine Plus (Invitrogen) according to manufacturer's instructions.

Platelet suspensions from LPS-treated animals were incubated for 30 minutes in water baths maintaining temperatures at either 37°C, 34°C or 31°C followed by exposure to thrombin (20 U/ml) and incubation with saturating amounts of the appropriate antibody.

For co-transfection, we equalized the total DNA amounts by adding the appropriate amount of pCDNA3.1 empty circular vector (Invitrogen).

Put a small amount (4-5 drofs) of the appropriate color indicator into the beaker.

The perception of the appropriate amount to eat will determine the amount served.

This study illustrates poor consumer awareness of appropriate portion sizes which tends towards an overestimation of the appropriate amount of food to consume.

Composites were prepared by mixing appropriate amounts of the two solutions inside 1.5 mL vials.