Sentence examples for amounts of templates from inspiring English sources

PCR cycles and amounts of templates were optimized for each primer set in pilot experiments.

Each sample was analyzed in triplicates, and the amounts of templates were estimated by linear regression against the known standard and normalized to internal control.

The calculation gives a relative ligation frequency for each analyzed sample, since it corrects for the differences in PCR amplification efficiencies, amounts of templates, and sizes of PCR products.

In addition, different amounts of templates at the beginning of the reaction produced proportionally different levels of final (end-point) fluorescence.

Overall, no difference was observed in the sensitivity, measured as call rate, for the different amounts of template DNA.

In this study, we prepared molecularly imprinted polymers with different amounts of template, porogen and cross-linker for the purpose of optimizing the binding capacity towards γ-oryzanol.

Filtration experiments showed that the imprinted hybrid membrane was able to adsorb higher amounts of template even in non-equilibrium dynamic binding conditions.

Using higher amounts of template (100 1000 ng) [16] and site-directed mutagenesis with primers to reintroduce the correct sequence [17] offered potential solutions that were not pursued here.

To determine the optimal amount of competitive cDNA to be added to each reaction, PCR was performed using either a fixed amount of template cDNA combined with decreasing concentrations of competitive cDNA or conversely, a fixed concentration of competitive cDNA combined with decreasing amounts of template cDNA.

Moreover, decreasing amounts of template DNA were used to investigate the limits of KRAS mutation detection.

Due to PCR amplification, minimal amounts of template DNA are required.