Because of the limited amounts of samples, P < 0.1 was considered statistically significant for all analyses.
For example, during clone selection, hundreds of clones should be analyzed quickly from limited amounts of samples.
Briefly, equal amounts of samples (exactly the same A260 units) were separated on a 15% SDS-PAGE gel.
Advantages of the PCR-ELISA method include convenient performance and the possibility to test rapidly large amounts of samples with a multitude of probes.
Compared to large-scale systems, the new microfluidic system uses smaller amounts of samples and reagents and performs fast diagnosis in an automated format.
For western blotting, equivalent amounts of samples were resolved on a 12% SDS-PAGE and the separated bands were transferred to a polyvinylidene difluoride (PVDF) membrane.
The uplink ICI is investigated in [14] by generating amounts of samples and then drawing a histogram without deriving a closed-form expression.
The lack of sensitivity associated with the need for detection of micro amounts of samples is probably one of the most frequently encountered problems in trace chemical analysis.
However, these techniques are time consuming and require expensive instrumentations, which is inconvenient for the routine analysis of large amounts of samples.
High-density protein microarrays allow to display a high number of analytes using minimal amounts of samples, constituting a promising high-throughput platform for the characterization of protein expression patterns, functional analysis and biomarker discovery.
Small amounts of samples and reagents could be injected into the diagnosis chips using the micro-pumps and the micro-pneumatic valves could effectively control the movement of the samples and reagents.
