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That is, the null expectation is equal amounts of reads from the paternal and maternal alleles.
Then, those huge amounts of reads were assembled into contigs by a de Bruijn graph-based method called SOAPdenovo [ 28].
As expected for high-throughput sequencing methods, which produce high amounts of reads, numerous deviations from the reference sequence were observed in the raw data.
Now, the unprecedented amounts of reads and increasingly longer inserts make haplotyping of an entire chromosome as a single connected block a distinct possibility.
Twenty-seven LTretrotransposonon families presented sufficiently large amounts of reads matching the 5′-end of the LTR region to be reliably aligned and analyzed through phylogenetics and molecular population genetics.
To test detection of Salmonella poisoning, varying amounts of reads simulated from a S. enterica genome (serovar Paratyphi A strain ATCC 9150) were spiked into the fecal dataset at the relative abundances of 1%, 0.1%, 0.01%, 0.001%, and 0.0001%, which corresponded to genome coverage depths ranging from 27× to 0.0027×.
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That book endured countless amounts of read throughs, and found its way into a second hand bookstore for someone else to enjoy.
Among the more than 100 million reads from high-throughput sequencing of the 6 libraries, there was also a large amount of reads that mapped to un-annotated genomic regions.
Both Bowtie and SOAP, due to their ungapped alignment processes, were able to map only a negligible amount of reads with an insertion or deletion.
The total amount of reads were 512,236 paired reads and 112, 079 single reads.
Second, misaligned reads represent a fraction of the total amount of reads generated and aligned.
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