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Only osteotomized animals accumulated lower amounts of differentiated cartilaginous cells on the anterior tibial side (Figs. 7a and 7b; Table 5).
Moreover, comparison of the regional accumulation of differentiated cartilage revealed that osteotomized animals accumulate lower amounts of differentiated cartilaginous cells on the anterior side where the surgical approach was located.
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Therefore, K5/14 pair is expressed in more than just corneal epithelial stem cells, and must also include a certain amount of differentiated corneal epithelial cells.
However, a small amount of differentiated pancreas cells can be found in Pdx1 deficient mice as reported in some experiments [29], [36], [37].
First, we performed IHC assays of teratoma sections with antibodies against the epithelial markers E-cadherin and Cytokeratin to identify the amount of differentiated epithelium in tumour samples.
Our simulations are consistent with the experimental observation that although LIF-BMP4 maintains ESCs, a low amount of differentiated cells are nevertheless produced.
In the case of SAT, the age (in weeks) of the mice and the amount of differentiated cells in the tissue seemed to affect the methylation profile of PPARγ promoter.
RORγ overexpression in 3T3-L1 cells decreases the amount of differentiated adipocytes as well as expression of the mature adipocyte marker adipocyte fatty acid binding protein (A-FABP; Fig 1B and C and Fig S1B of Supporting information).
During normal homeostasis as well as after injury, lung epithelial stem cells need to give rise to the right amount of differentiated progeny while maintaining their self-renewing ability depending on the physiological context.
This is probably due to the diffuse distribution of N-surv-positive cells in both tumour types, whereas the high amount of differentiated cells in WD and MD tumours, which fail to express survivin, contribute to the decrease in the percentage of N-surv-positive cells.
A few BrCSCs locate inside the core of tumorsphere (viewed as an atomic nucleus) and a large amount of relatively differentiated progenies surround the outer layer (viewed as electrons).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com