Sentence examples for amounts of bromophenol from inspiring English sources

and trace amounts of bromophenol blue.

The equilibrated strip was applied onto the top of a 12.5% SDS-PAGE gel and sealed with 1% agarose prepared in SDS-Tris-glycine buffer with trace amounts of bromophenol blue as a tracking dye to monitor electrophoresis.

To 110 µg of protein sample (10 mg/ml), 128 µl of 1.1x ZOOM 2D protein solubilizer 1, 0.7 µl DTT (2 M), 0.3 µl Bio-Lyte 3/10 carrier ampholyte, and trace amounts of Bromophenol Blue were added, mixed and rehydrated onto a ZOOM strip (pH 3 10) for 1 h at room temperature.

Prior to rehydration of the immobilized pH gradient strips, trace amounts of bromophenol blue was added to samples.

Pellets were resuspended in 200 μl of rehydration buffer (7M urea, 2 M thiourea, 1% DTT, 1% CHAPS, 1% Triton X-100, 1% Ampholine pH 3-10, and trace amounts of bromophenol blue).

Equilibrated strips were placed horizontally on top of NuPAGE 4-12% Bis-Tris gels (Invitrogen) and secured in place by covering it with 0.5% agarose gel (w/v) made up in running buffer and containing trace amounts of bromophenol blue.

Samples were withdrawn, mixed with the same volume of 2xSDS-PAGE loading buffer (250 mM Tris-HCl (pH 6.8), 4% (wt/vol) SDS, 30% (vol/vol) glycerol, 10 mM DTT, a trace amount of bromophenol blue) and incubated at 37 °C for 10 min.

Finally, the beads were resuspended in 2× sample buffer (100 mM Tris-HCl pH 6.8, 20% glycerol, 4% SDS, 0.2 M DTT and a trace amount of bromophenol blue) and boiled for 5 min before being loaded on a gel.

A small amount of bromophenol blue was added to eluate and proteins were separated by SDS-PAGE followed by Western blotting for IGF-IR or GAPDH as described above.

The protein (2 mg) was solubilized in rehydration buffer containing 6 M urea, 2% CHAPS, 20 mM DTT, 0.5% IPG buffer, and a trace amount of bromophenol blue.

For each lane, 4 μL of the reaction mixture was mixed with an equal amount of 2× native loading buffer containing a small amount of bromophenol blue in 2× TAE/Mg2+ buffer and 10% glycerol.