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Comparing plasma DNA levels to DNA amounts isolated from 1 ml serum, we could ascertain that plasma DNA isolations of control individuals provide 57% (P = 0.002, Student t-test) of the DNA amount detected in serum of the same blood donors.
Amounts isolated from different tissues varied by a factor of 3. The purified substance reacted with ninhydrin and a few other reagents for amino groups.
However, this reduced DNA amounts isolated with MBD loaded beads did not affect the serum DNA recovery, because the agarose gel image based analyses of amplifiable serum DNA fragments was not influenced by the methylation status of DNA fragments.
We nevertheless verified that differences in concentration were not affecting the results: in experiments using equivalent Chl amounts isolated core and antenna moieties were more sensitive to high light treatment with respect to whole PSI-LHCI complexes (see Additional file 3).
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Because of the limiting RNA amount isolated from muscle biopsies used for microarray analysis, only a few samples (6 cases) were used for confirmation with quantitative RT-PCR experiments.
For each sample, a mucus score was assigned during endoscopic examination according to Gerber et al [63] (Score 0: no visible mucus, score 1: small amount, isolated flecks, score 2: moderate amounts, multiple larger blobs, and score 3: large amounts visible, larger blobs at least partly confluent).
The yields reported for the syntheses of the copper(I) amide complexes were calculated from the amount isolated after purification.
Due to the small amount isolated, and the pilots trials performed in our laboratories, the compounds were evaluated pharmacologically in single dose.
The total DNA amount isolated from at least 250 μl of whole saliva can be sufficient for whole genome analyses requiring 1.5-3 μg DNA.
The mean DNA amount, isolated from 1 ml serum of 60 control individuals using the silica membrane based approach was 22.8 ± 25.7 ng/ml (mean ± SD ., ranging from 0.3 ng - 39.1 ng.
With the starting amount of cells kept equal across experiments, a time dependent decrease of the total amount of isolated total RNA was obtained.
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