The phrase "amount were performed using" is not correct in written English.
It seems to be a grammatical error, likely due to a mismatch between the subject and verb forms.
Example: "A significant amount of tests were performed using the new software."
Alternatives: "number were conducted using" or "quantity were executed using".
Quantification of the relative mRNA amount was performed using the ΔCt method with the 7500 Software v2.0.6 (Applied Biosystems, USA).
Prediction of evaporation amounts is performed using Support Vector Regression (SVR) originated from Support Vector Machine (SVM).
Transfections using a constant amount of plasmid DNA were performed using Lipofectamine™ and PLUS™ reagents (Invitrogen), according to the manufacturer's protocol.
Investigations of the amount of water in MCC were performed using derivatograph Q-1500D.
As 5 μg of enzyme was the smallest amount that led to H2S synthesis that was well above the detection limit, all subsequent experiments were performed using this amount of protein.
To test these possibilities, anti-FLAG co-immunoprecipitation (co-IP) experiments were performed using equal amounts of total protein extracted from transgenic plants expressing either the wild-type or active site mutant versions of the NRPD1-FLAG and NRPE1-FLAG transgenes (Figure 2C).
Since the maximum decolorization of BGY is observed with 6 g/L of ZnO-TiO2 photocatalyst, the other experiments were performed using this amount of the composite.
To determine the optimum amount of input DNA, analytical amplifications were performed using serial dilutions of linker-ligated DNA: spanning four orders of magnitude (from 1000 to 0.064 pg/µl).
PCR amplifications were performed using equal amount of synthesized cDNA with gene specific sense and antisense primer sets using Phusion High-Fidelity DNA Polymerase (New England Biolabs Inc., MA).
To quantify the amount of Bcl11b expression, Western blots were performed using an antibody against human BCL11B which displays some cross-reactivity to mouse Bcl11b.
