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3T3-L1 cells were grown on glass coverslips, differentiated into adipocytes, and stimulated or not with insulin for 20 min. Plasma membrane sheets were prepared, immuno-stained with anti GLUT4 or anti TfR antibodies, and quantification of their amount was performed as previously described in [22], [43] using WGA labeling for normalization to the amount of membranes.
A sensitivity analysis for the Ag amount and the total ADA amount was performed against model parameters.
Quantification of the relative mRNA amount was performed using the ΔCt method with the 7500 Software v2.0.6 (Applied Biosystems, USA).
Among 51 patients (male: 20, female: 31) using a Diskus (fluticasone propionate, salmeterol/fluticasone combination) "confirmation of counting of residual amount" was performed by 90% of males and 87% of females, while "correct operation of the lever" occurred for 90% of males and 81% of females, with no significant difference between the sexes.
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Estimates of transcript amount were performed using the comparative threshold cycle method.
Quantification of the lipid amounts was performed by scanning the plates with a Hewlett Packard Scanjet 4500c and by integrating the density areas with Tina version 2.09 software.
Normalization of the samples to the internal standard and the quantification of carotenoid amounts was performed as described previously [ 38].
Quantification of relative protein amounts was performed by densitometric analysis using ImageJ software (NIH), normalized to a loading control protein, growth factor receptor-bound protein 2 (GRB2).
Prediction of evaporation amounts is performed using Support Vector Regression (SVR) originated from Support Vector Machine (SVM).
Electrophoresis of equal amounts of total protein was performed on SDS-polyacrylamide gels.
To determine the proportion of ganglioside GD2 content to the total ganglioside amount, densitometric analysis was performed for ganglioside fractions isolated by HPTLC from selected cell lines.
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