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The fluorescence intensity corresponding to the intracellular lipid amount was determined at the peak of the corrected spectrum.
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DNA amount was determined by optical density at 260 nm (OD260) using an Eppendorf BioPhotometer 6131 (Hamburg, Germany).
The amount was determined spectrophotometrically.
The lower amount of coke was determined at 350 °C, the higher at 450 °C.
The relative amount of SSB was determined at various DMSO concentrations.
For the control release studies of 5-FU from the loaded nanocomposite films, known weights were placed in a measured volume (50 mL) of 7.4 pH phosphate buffer solution at room temperature and the released amount of 5-FU was determined at different time intervals by recording the absorbance of the release medium using the UV vis spectrophotometer [48].
For the longitudinal images acquired over tested segments, the amount of echogenic bands/densities was determined at three separate layers: dermal, subcutaneous, and perimuscular zones (see Figure 3).
As a control, the amount of Pi generated was determined at various concentrations of SwHaCE.
The amount of remaining DPPH was determined at λmax 517 nm.
The amount of PNP released was determined at 400 nm with an extinction coefficient of 19,608 M−19,608.
Subsequently the amount of free rhodamine was determined at a microplate fluorescence reader (TECAN spectrafluor, Switzerland).
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