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The precipitate containing unloaded drug was dissolved in 50 % THF solution, and its amount was analyzed by UV visible spectrophotometry at 330 nm.
cDNA amount was analyzed by real-time quantitative PCR using SYBR Green I/Rox in Chromo4 Real-Time PCR Detector (Bio-Rad).
Specific protein amount was analyzed using the standard procedure of western blot analysis.
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On the basis of the real coal seam conditions, the variation rules of the stress, leakage ring, and air leakage amount were analyzed through numerical simulation, and the influence factors of air leakage amount were also investigated to provide the theoretical basis for the sealing technology.
Since the logical variables have been introduced, the optimization problem including obstacle avoidance and system physical limits as prioritized inequality constraints is termed as MIPC method of space robots, and its computational complexity as well as possible strategies for reducing calculation amount are analyzed.
Standard regressions between ω and Transposable Element (TE /repeat amount were analyzed for each gene separately and statistical significance was determined using the R package.
Dispersion obtained for fracture mean values among the different amounts was analyzed by using a fracture surface analysis and the amount and distribution of fibers.
Equal sample amounts were analyzed by Western blotting, and signals were standardized against an endogenous 60 kD protein not affected by transgene expression, but reliably cross-reacting with the α-Penta-His antibody (Fig. 1A).
Under these conditions a higher amount of protein was seen in the cell extract as equal protein amounts were analyzed.
Samples were normalized to Gapdh and relative quantitative amounts were analyzed using the cycle threshold method (2^-ΔΔCt).
To verify if the phenotypic indications for BR deficiency were correlated with changes in endogenous BR levels, BR amounts were analyzed in aerial plant parts of a line with strong UGT73C6 expression (UGT73C6oe/2-10) and compared to wild type by GC-MS in two independent biological experiments.
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