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Equal amount proteins with appropriate volume of loading buffer were loaded onto SDS-PAGE.
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aExpressed as quantity per total protein amount.
= [(Total protein amount − Free protein amount)/Total protein amount] × 100%.
NAMPT amounts in cell lysates and supernatant fractions were normalised to total protein amount (BCA Protein Assay, Thermo Fisher Scientific, Bonn, Germany).
Calculate total protein amount of each sample.
Equal amounts of total protein were loaded in lanes 1 2.
All lanes contain equal total amounts of proteins (3 μg).
Data were normalized to amount of proteins.
The amount of proteins decreased in all but CL leaves.
The amount of proteins was quantified by the Lowry method.
The amount of proteins was estimated as N × 5.7.
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