Sentence examples for amount of vector and from inspiring English sources

From these results, it can be determined what amount of vector and fragments that should be used and how many ligations that are required to make the library.

On the contrary, AAV has relatively low transduction efficiency, and it requires a larger amount of vectors and more time for transduction and transgene expression.

Thus, several ligations with varying amounts of vector and DNA fragments, respectively, should be made and electrotransformed into E. coli.

For zebrafish eaf1 and eaf2/u19 promoter assays, zebrafish embryos were injected with the indicated amounts of vectors and the Renilla luciferase reporter as an internal control.

For human Eaf1 and Eaf2/U19 promoter assays, 293 cells grown on 24-well plates were transfected with the indicated amounts of vectors and the Renilla luciferase reporter as an internal control, using Lipofectamine 2000.

The amount of vector DNA and the ratio of insert to vector DNA were examined.

The total amount of vector transcripts, and the amount that is contiguous with sequences 3' of the polyadenylation signal, were then determined to allow the frequency of polyadenylation signal read-through to be measured.

Directional ligation was performed in 10  μl reactions containing 1  μl T4 DNA ligase, 1  μl 10 × ligation buffer (Promega), 100 ng insert and the appropriate amount of vector to give 3 : 1, 1 : 1 and 1 : 3 molar ratios of insert to vector.

This can be done by varying the amount of vector DNA 10-200 μg/ml) and fragments (10-100 μg/ml) in a number of ligations.

To evaluate the effect of Sall2 and p53 in Noxa promoter activity in H1299 and HCT116 (p53-null) cells, we used 2  μg of each Sall2, p53 or both, and equivalent amount of vector control for comparative analysis.

For vector controls, the same amount of vector (instead of the new isoform) was injected along with GABAB1a, GABAB2, GIRK1, and GIRK2 into the control oocytes.