Quantitative PCR showed that transcripts from the LCR HSs are sequentially increased after transcriptional induction; although the amount of transcripts was lower than the mRNA amount of the βmajor globin in uninduced MEL cells.
Changes of abundances of transcription factors cause changes in the amount of transcripts of their target genes.
Accordingly, the amount of transcripts corresponding to Dnmt1 decrease significantly as fibroblasts senesce, while Dnmt3b transcription is up-regulated, suggesting the loss of methylation results at least in part from changes in Dnmts transcriptional regulation [ 24].
Specific primers for each ADA member were designed, optimized semi-quantitative RT-PCR experiments were conducted and the relative amount of transcripts was determined.
Therefore, a large amount of transcripts will be missing from these EST libraries.
Notably, splicing repression was associated with decrease in exon included and concomitant increase in exon excluded transcripts, relative to the total amount of transcripts.
Briefly, threshold cycle (Ct) values were converted to 2−Ct in order to be proportional to the amount of transcripts in the samples.
Indeed, in microarray-based experiments, a lower hybridization can result either from a reduced amount of transcripts or from divergence in the sequence of the gene [20].
The amount of transcripts of CA IV mRNA in corpus epididymidis is comparable to that of the kidney, which served as reference.
Unlike promoter activity, which primarily regulates the amount of transcripts produced by a gene, alternative splicing changes the structure of transcripts and their encoded proteins.
Separate Rsd titration experiments with the Eσ70 holoenzyme revealed that 250 nM Rsd are already sufficient to reduce the amount of transcripts for the σ70-dependent promoters tac, rrnB P1 and the RNA1 promoters to a similar extent.
