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If there are limitations to the amount of starting RNA available, then the more starting material used the better (within this range examined).
(C) Copy number profiles of mESC gDNA through TTAS, with different amount of starting material.
Hence handing small amount of starting DNA material is still challenging.
We performed a titration experiment to better test the efficiency of TTAS on small amount of starting material.
The quality and amount of starting RNA was confirmed using Bioanalyser (Agilent).
Finally, with the amount of starting material used, fOSGN2 could not be clearly identified in MBC fractions.
We used a large amount of starting material, i.e. ∼1000 hand-dissected Drosophila brains in each immunoprecipitation experiment.
When the amount of starting material is limited, Whole Genome Amplification before sonication could provide the necessary DNA quantity.
Second, the amount of starting material required was also prohibitively high in the initial stages.
Next, we investigated whether the amount of starting material affected sequence data quality.
There was no clear relationship between the amount of starting RNA and the sscDNA yield.
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