Exact(2)
The amount of spacer #1-containing RNA including the sequence found on the sense strand of hisS, detected by primer MA0327, was not significantly different from the amount of spacer #1-containing RNA corresponding to the antisense of hisS, detected by primer MA0326.
Therefore, the limiting factor for interference with hisS may be the amount of one or more Cas proteins, or the leader sequence, rather than the amount of spacer #1 transcript.
Similar(58)
The number of GTAG-1 TT dimers in the two species is comparable, but the relative amount of spacers with complementary ends is significantly different.
PAMs were not clearly detected in any of the four CRISPR types (supplementary fig. S13, Supplementary Material online), which may be due to the reduced amount of spacers exhibiting high nucleotide similarity to the sequences in the databases.
Use small shims or other spacers to allow a small amount of space between the bottom of the tile and the lip of the shower pan.
Spacers are shaped like "plus" signs, but for the bottom row of tiles, you must trim off the descending appendage to leave the proper amount of space for grout.
The small decrease in the amount of hisS RNA in the presence of spacer #1, comparable to the decrease in the amount of transcript for a control housekeeping gene (hisZ) that accompanies the growth defect, is unlike the extent of decimation that one would expect if spacer #1-containing RNA catalyzed degradation of hisS RNA.
Furthermore, this inhomogeneity combined with the above-listed inter-individual factors makes it difficult to assess with any accuracy the total amount of antibiotic released from spacers in vivo.
Despite the fact that the beads were impregnated with half of the amount of antibiotic that the spacers were, in the first days the beads released greater amounts of both agents due to their larger surface area (Anagnostakos et al. 2006).
Start with the center gridline(s) and place additional tiles leaving the appropriate amount of room for the spacers, which will later be grout lines.
The worker, Leo T. Lindner, a drilling fluid specialist for M. I. Swaco, testified at a government hearing in the New Orleans suburb of Kenner that BP had given him permission to use an unusually large amount of a fluid called a "spacer" when conducting tests of the blowout preventer, a last-ditch safety mechanism that failed during the disaster.
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