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Different concentrations of carboxyl-modified NaYF4 30%Yb0.5%Tm UCNPs ranging from 0.25 to 2 mg mL−1 were conjugated to a fixed amount of reporter antibody.
GFPmut3.1 variant can be detected after a short lag-phase of only 10 min, it shows a high fluorescence yield in relation to the amount of reporter protein, a good signal to noise ratio and a low detection limit.
Luciferase assays measure the amount of reporter protein available at the time of assay and reflect the combined effects of regulation at the transcriptional and translational levels.
For each transfection experiment, 2BS cells were seeded at 12-well plates and grown for about 24 h until they were above 90% confluent, then transfected with an equal amount of reporter plasmid (1.6 µg) and 0.32 µg of pRL-CMV (Promega) as transfection efficiency control, using lipofectamine 2000 (Invitrogen) and following the manufacturer's indications.
Levels of mCherry can thus be used to normalize for the total amount of reporter per cell.
The amount of reporter plasmid (pMN-Luc) used was 300 ng per well and the effector plasmids (pFA Gal4 fusions with RD segments) were 30 ng per well.
Similar(48)
ii) Similar amounts of reporter luciferase are produced in the global reporter mice and in the mice reporting production of IFN-β by monocytes/macrophages and neutrophilic granulocytes.
Control wells included identical amounts of reporter and helper plasmids but lacked the L-encoding plasmid.
However, from 24 to 96 h, the mRNA amounts of reporter gene were decreased with time).
Interestingly, the inclusion of additional binding sites added only modest amounts of reporter activity.
This finding raises the possibility of producing sufficient amounts of reporter virus constitutively.
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