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However, since NiV pathogenicity is extremely strong, the lower amount of replicated rNiV(W−) in the body seemed to be sufficient to kill the animals.
After dialysis and concentration, the total amount of replicated DNA in the samples was determined from the specific activity of C-labeled DNA.
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This finding confirms that the vaccine strain replicates in the lymphoid tissues; while the amount of replicating virus is presumably low.
For each primer set (Table 2), an equal amount of replicating DNA from samples representing seven 1-h windows of the S phase was tested and the approximate time of replication was determined as described in the Methods section.
Equal amounts of replicating DNA from samples representing seven hrs of the S phase were used as template for PCR reactions.
As for the support of the trees, we expect it to be relatively low given the sample size and the amount of replicates, and our focus here is on the qualitative estimate of how different distances perform in the problem of tissues clustering. 5.
A simple explanation of this is that replicases must spend some finite amount of time replicating other molecules, during which the replicases themselves are not replicated, whereas those that do not replicate other molecules (parasites) can spend more time being replicated.
In contrast, the cats in this study showed no clinical signs of intraocular inflammation despite the presence of relatively large amounts of replicating virus.
Our parallel code enables the phylogenetic study using a statistically robust amount of bootstrap replicates for large protein families using the distance and parsimony methods as well using a statistically robust amount of bootstrap replicates for moderate sized protein families using the maximum likelihood method implemented in PHYLIP.
The table indicates the flower type, the denomination of the genotype or variety, the country of origin (if known) and the amount of measured replicates n.
Given the limited amount of mRNA, replicates were only performed for the DASL assay due to its relative novelty as compared to the IVT assay.
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CEO of Professional Science Editing for Scientists @ prosciediting.com