The phrase "amount of raft" is not correct and does not convey a clear meaning in written English.
It may be intended to refer to a quantity of something related to a raft, but the phrasing is awkward and unclear.
Example: "The amount of raft needed for the project was underestimated, leading to delays."
Alternatives: "quantity of raft" or "number of rafts".
It is interesting that, in U87MG cells, both TAXOL and Vinblastine significantly reduced the total amount of raft-associated BAD, the phosphorylating target of Akt, without changing the amount of phosphorylated BAD in the lipid rafts.
It is interesting that, in the HCC tissues, the expression of raft-associated mTOR was significantly increased, but the amounts of phosphorylated BAD were significantly reduced in the lipid rafts, without changing the total amount of raft-associated BAD.
The amount of lipid raft coverage, total number of cell surface receptors, GTP hydrolysis rate constant and ligand concentration were shown to affect the amplification ratio.
Cells were then stained with Alexa488-conjugated Cholera Toxin B subunit (1 100, Sigma Aldrich) to examine the amount of lipid raft and APC-conjugated TLR4/MD2 complex (MTS510, eBioscience) or isotype control to examine TLR4/MD2 cell surface expression in 0.1% BSA, 5 mM EDTA in PBS.
Consequently, PAF antagonists had similar effects to squalestatin on the trafficking of Aβ42 in that they reduced the amount of Aβ42 within rafts.
Following tunicamycin treatment, the amount of AC8 in rafts was dramatically reduced (Fig. 3 A ) and the N-glycosylation-defective mutant of AC8 is mainly, if not exclusively, concentrated outside of rafts (Fig. 4 C ).
Together, the relative amounts of the raft-typical sphingophospholipid classes, SM and IPC, were increased in flagella (17%) compared to whole cells (7%) (Fig. 7).
Overall, the total amount of collagen impacted upon RAFT TE optical properties (i.e. RAFT TEs produced using 2.4 ml of 1 mg/ml collagen or 1.2 ml of 2 mg/ml collagen (both a total of 2.4 mg collagen) were comparable).
A possible explanation for this finding is that there is a change in the amount and/or size of rafts or their ability to become associated with the endocytic machinery.
