The phrase "amount of purifying" is not standard in written English and may cause confusion.
It could be used in contexts discussing the quantity or degree of purification in a process, but it is better to clarify the intended meaning.
Example: "The amount of purifying required for the water treatment process was higher than expected."
Alternatives: "degree of purification" or "level of purification".
However, non-conservative deletions are in addition subject to a significantly larger amount of purifying selection compared to non-conservative events, and a similar statement is likely to be true also for insertions.
We also report fairly strong purifying selection removing up to 85% of all non-synonymous (amino acid changing) mutations and marginal evidence for heterogeneity in the amount of purifying selection and positive selection among gene categories.
We reasoned that if the density of polymorphism was governed by the amount of purifying selection acting on each site, then SNP density would be correlated with divergence across species, in accordance with expectations under the Neutral model (Kimura 1982).
However, these genes are, as expected, experiencing a fair amount of purifying selection with a mean dN/dS = 0.097 (std. dev. = 0.091), with a range from 0 to 0.38.
Model comparison using differences in AIC provides suggestive evidence that, despite low level of polymorphism compatible with a rather small long-term effective size, a substantial amount of purifying selection constrains amino acid changing polymorphism.
The second hypothesis presumes that in addition to the previous "selectively neutral" explanation at least part of the observed strong bias may reflect a higher amount of purifying selection associated with non-conservative indels due to their larger number of effective changes in the protein sequence compared with conservative events.
However, a sufficient amount of purified protein is needed for the in vitro screening of potential TNF-α inhibitors.
We present here a protocol which can be used easily to obtain milligram amount of purified rat 5-HT2c receptors.
S-NaR1 was purified to homogeneity in one step by immobilized metal affinity chromatography (IMAC) and total amount of purified protein per run of fermentation was ∼180 mg.
The determination of optimal operating policies is aimed to maximise the amount of purified product(s) and thereby achieve better column performance whilst keeping the matrix cost constant.
Standard assay mixture (200 μL) was composed of 200 mM PBS (pH 7.4), 5 mM alkene substrate, 0.2 mM NADH, and an appropriate amount of purified enzyme.
