Protein levels of β−tubulin were also assessed to ensure equal amount of proteins.
Protein levels of histone H3 were also assessed to ensure equal amount of proteins.
The amount of proteins from each fraction in the input represent 10% of total lysates used for immunoprecipitation.
Immuno-precipitations were set up, overnight, with desired amount of proteins and antibodies (antibody amounts have been listed above).
Cancer cells produce an unusual amount of proteins at a rapid rate to support division, growth and invasion.
The irradiated samples adsorbed significantly greater amount of proteins than pristine.
As Sestrin2-deficient cells continue to translate a large amount of proteins during ER stress, they are highly susceptible to ER stress-associated cell death.
In a second set of tests, the cells were screened to see if there were any changes in the amount of proteins expressed – a process known as proteomics.
Each sample with equal amount of proteins (as the loading control) was separated on 12% SDS-PAGE before transferred onto a nitrocellulose membrane.
The amount of proteins was quantified with densitometry analysis using Image J. Detailed information of antibodies used for Western Blot analysis are available in Supplementary Information.
Moreover, the amount of proteins also played a key role over the morphology and crystalline of products.
