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The World Health Organization (WHO) provides stringent requirements for effluent based on the amount of pathogens present within the system.
The amount of pathogens in the substrate (waste and feces) is decreased, so that it can be reused as fertilizer (Bond and Templeton 2011).
It is essential that CL-care solutions should be able to sufficiently decrease the amount of pathogens in order to decrease the risk of CL-related infections [13].
For aspirates, the amount of pathogens > 10/ml was considered significant.
Bacteriophages have been suggested as an alternative approach to reduce the amount of pathogens in various applications.
Implementing a root growth stimulation when using R-plants could so increase the "trap plant" effect and thus decrease the amount of pathogens in the soil.
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The initial amount of pathogen DNA is determinable by the assessment of the PCR crossing point [20], [21].
EcO157 present in agricultural environments are expected to be stressed, and possibly non-culturable, increasing the problem of measuring accurately the amount of pathogen in samples [31], [32].
Studying pathogens inside host tissues poses a further complication, as the amount of pathogen RNA often becomes vanishingly small compared to host RNA.
Another advantage of real-time PCR is that, unlike conventional PCR, it offers the potential to quantify the amount of pathogen DNA present in a clinical sample.
High levels of SA and its glucoside were also found in the xylem sap of V. longisporum-infected B. napus and were correlated to the degree of stunting and the amount of pathogen DNA [ 23].
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