Sentence examples for amount of mRNA generated from inspiring English sources

The average amount of mRNA generated for DC transfection was 5.3 ± 2.3 × 10 μg.

It is well known that the total amount of mRNA generated by tumor cells, for example, is much higher than the amount generated by normal cells.

Both histones H2A.J and H2A.X exhibited medium levels of gene expression with only marginal differences in the amounts of mRNA generated from these genes, when compared with β-actin gene expression.

For the library, equal amounts (250 μg) of total RNA from the four time points were pooled to form the mRNA (2 μg) tester, while mRNA generated from the roots under normal conditions was used as the driver.

The amount of cDNA generated from mRNA was analysed in a RT-qPCR assay using a plasmid standard, allowing for detection and estimation of the mRNA expression levels produced from individual transgenes.

While oligonucleotide arrays measure the amount of mRNA in a single sample, gene expression data generated using cDNA array platforms are ratios of expression values in experimental samples over those in a reference sample.

This observation can be attributed to the markedly higher amounts of hexon and protease mRNAs generated from the particularly strong MLP, in comparison with the mRNA levels of the other genes.

The amount of mRNA was then quantitated by extrapolation from the standard curve generated with the internal standard.

This method can readily be used to generate shRNA libraries from a small amount of mRNA and thus can be used to create cell- or tissue-specific libraries.

Although the direct repressive trans-acting action of miRNAs is to cause a net reduction in the total amount of protein generated from their target mRNAs, developmental and physiological processes have combined this with the flexibility of spatial and temporal regulation of both the miRNAs and their targets to employ miRNAs in a range of regulatory roles.

The amount of mRNA in each sample was normalized with the amount of 16S rRNA.