The phrase "amount of insert" is not correct in standard written English.
It may be intended to refer to a quantity of something, but it lacks clarity and proper context.
Example: "The amount of insert needed for the project is still being determined."
Alternatives: "quantity of insert" or "volume of insert".
The amount of insert for ligation was calculated according to following formula: Insert mass (ng) = 6× Insert length (bp) / Vector length (bp) × Vector mass (ng).
The standard SLiCE reaction solution comprised the following components: 10 ng linear vector (PCR amplified or restriction enzyme-digested), an appropriate amount of insert DNA (1 1 to 3 1 molar ratio of insert to vector), 1 μL 10× SLiCE buffer, 1 μL SLiCE extract, and sterilized distilled water to a total volume of 10 μL.
We first began by titrating the concentration of pR primer from 20 μM to 0.1 μM due to the following considerations: (1) to control the amount of insert in an suitable level to anneal with pUC19 and generate linear sequences and (2) to permit KOD Plus polymerase to focus on amplifying the linear fragment in an exponential manner.
50 μg of vector pCANTAB6 was successively digested with 80 units of NcoI and NotI for at least 6 h each, purified then quantified on gel. 5 μg of linearized pCANTAB6 was ligated with an equal molar amount of insert (0.84 μg) in 500 μl at 16°C using 50 Weiss units of T4 DNA ligase.
The amount of inserted lithium and, also, the reversibly extracted lithium, depends on the quality of MoS2−xIy NTs bundles and their prior treatment.
Comparing their electrochemical properties with layered 2H-MoS2, one finds a significant increase in the amount of inserted lithium and a decrease by about 0.7 V in the potential at which lithium insertion takes place.
An appropriate amount of inserted 1-D MWCNTs not only effectively prevent the aggregation of rGO sheets but also act as bridges to increase multidirectional connections between 2-D rGO sheets, resulting in a 3-D hierarchically porous structure with large surface area and excellent biocompatibility for rich bacterial biofilm and high electron transfer rate.
As the amount of inserted data increased, the response time of the deletion operation increased.
When the amount of inserted data increases, the Skip-Octree shows its performance advantage.
As shown in Fig. 7, when the amount of inserted data is small, Skip-Octree consumes nearly the same amount of time as does the traditional Octree.
Timescale is riding the idea that companies are doing an increasing amount of inserting information into their databases, which typically happen in order — hence making sense to order it in some kind of time series.
