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RTK quanta with different sizes could also result from differential phosphorylation of Hrs by RTKs (Row et al., 2005), given that the relative amount of Hrs on endosomes depends on its phosphorylation state (Urbé et al., 2000).
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These RLU/CO ratios also provided an estimate of the amount of hr-HPV DNA present in the specimens, that is, the semiquantitative viral load.
A stepwise multiple regression analysis identified the eGFR (ß = 0.482) and 24-hr urinary protein (ß = 0.351) as predictive variables for the log-transformed value of the amount of 24 hr urinary excreted Klotho (Total R = 0.284, p < 0.001), while only the serum calcitriol (ß = 0.267) was selected as an explanatory factor for the serum Klotho concentration (Total R = 0.0713, p < 0.01).
The log-transformed value of the amount of 24 hr urinary excreted Klotho significantly correlated with the eGFR and serum calcitriol.
The amount of 24 hr urinary excreted soluble Klotho trended towards lower values with advancing stages of CKD (overall p < 0.001).
The use of the random urine Klotho/Cr ratio as a surrogate for the amount of 24-hr urinary excreted Klotho needs to be evaluated more carefully.
The use of random urine Klotho/Cr as a surrogate for the amount of 24-hr urinary excreted Klotho needs to be evaluated more carefully.
A significant association between the log-transformed value of the amount of 24 hr urinary excreted Klotho and that of the serum soluble Klotho levels (r = −0.403, p < 0.01) was also observed.
The estimated glomerular filtration rate (eGFR) was significantly correlated with the log-transformed values of the amount of 24 hr urinary excreted Klotho (r = 0.407, p < 0.01) and the serum Klotho levels (r = 0.232, p < 0.01).
However, a stepwise multiple regression analysis identified eGFR to be a variable independently associated only with the log-transformed value of the amount of 24-hr urinary excreted Klotho but not with the log-transformed serum Klotho concentration.
Despite the strong correlation between random urine protein-to-creatinine ratio and the 24 hr urinary protein excretion (r = 0.834, p < 0.01), a moderate linear association was observed between the log-transformed value of the amount of 24 hr urinary excreted Klotho and that of the urinary Klotho-to-creatinine ratio (Klotho/Cr) in random urine specimens (r = 0.726, p < 0.01).
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