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This involved subtraction analysis (see Supporting Information S1 for detailed description), according to which "loading capacity = [the total amount of GEM introduced for particle soaking – (non-entrapped GEM in the supernatant before pore sealing + GEM in the washings after pore sealing)]/[the total amount of MSNP] × 100%.
We defined loading capacity = [the total amount of GEM (m0) – (m1 + m2)]/[the total amount of MSNP (mNP)] × 100%.
A series of GEM/PTX mixtures were prepared in which we initially used a fixed amount of GEM plus different amounts of PTX to yield GEM/PTX ratios over the range of 100 1 to 0.2 1.
A series of GE M/PTX LB-MSNPswere prepared in which we used a fixed amount of GEM (25%, w/w) in the presence of 0.25 5 wt % PTX to yield GE M/PTXratios of 100 1, 10 1, and 5 1.
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The last pony, needed to finish the game, used to cost 500 gems - the equivalent of £34 ($44) - and some users complained on forums that it was extremely difficult and took too long to get the amount of gems required without paying.
Recycle your den to get a good amount of gems.
Getting into one of the top three Clans can earn you a significant amount of Gems.
Keep doing this until you get the amount of gems you want!
Play as many games as you can to get the maximum amount of gems.
A recommended amount of gems for your spree is 2,500 to 5,000.
By removing obstacles, you will receive a random amount of gems and maybe 10 gems once every so often.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com