The solid lines in Figure 4 show the effect of increasing amounts of fractionation on the apparent amount of rearrangement, a gross distortion.
A high amount of cellulose fibrous fraction (70.4%), with 63.1% fiber cell content and 65.6% cellulose content, was produced from the fractionation process.
However, after subcellular fractionation, the amount of actin in the soluble (depolymerized actin) and insoluble (polymerized actin) fractions showed a modification in the polymerized/total actin ratio during encystment).
Defective GPAT4 localization to LDs with Arf1/COPI depletion was also evident in subcellular fractionation experiments, where the amount of GPAT4 in the LD fraction was greatly diminished in the absence of βCOP.
We carried out extensive simulation experiments to assess how the process of fractionation impacts the apparent amount of rearrangement.
Membrane and nuclear fractions of HPAEC were isolated by cell fractionation as described in Materials and Methods and the amount of TIMAP in the fractions was detected by Western blot.
However, a generally valid, quantitative prediction of how geochemical parameters vary as a result of phase fractionation is very difficult, as this will depend not only on the pressure and temperature of fractionation, but also on the composition and amounts of the oil and gas that are involved.
This may be the result of further cleavage and subsequent degradation of the N-terminal product, presumably due to the cell fractionation conditions and amount of exogenous Calpain1 present.
