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The amount of formazan generated is directly proportional to the cell number over a wide range.
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After incubation for 5 min at ambient temperature, the absorbance at 562 nm was measured against an appropriate blank to determine the quantity of formazan generated.
The amount of formazan dye generated by cellular dehydrogenase activity was measured by absorbance at 450 nm with a microplate reader.
The amount of formazan dye generated by the dehydrogenases was directly proportional to the number of living cells.
The amount of MTT-formazan generated was measured as the absorbance by using a microculture plate reader at 540 nm.
After 4 h of incubation at 37°C, the MTT solution was removed and 100 μL dimethylsulphoxide (DMSO) was added to resolve the formazan generated from MTT.
The density of formazan deposits also reflected the amount of superoxides generated locally.
The amount of formazan present is proportional to the number of viable cells, because only living cells will reduce MTT to blue formazan.
The amount of formazan so produced is directly related to cell viability [70].
The amount of formazan was quantified using ELISA reader at 450 nm.
The amount of formazan so produced is determined by using microplate reader data.
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