Sentence examples for amount of dna template from inspiring English sources

Ratio of outer and inner primers, amount of DNA template, reaction temperature and time were optimized.

The lowest amount of DNA template of H. walsbyi DSM 16854 that the IMFET biosensor could detect was 125 fg.

The conventional genotyping method used for this polymorphism (multiplex PCR with fragment detection by gel electrophoresis) is laborious and requires large amount of DNA template.

With 30 cycles, the results showed that the optimal amount of DNA template for this multiplex ranges from 250 pg to 2 ng and the lowest detection threshold is 125 pg (as low as 63pg for ABO loci).

By first amplifying the DNA sequence and then analyzing the product, quantification was exceedingly difficult since the PCR gave rise to essentially the same amount of product independently of the initial amount of DNA template molecules that were present.

The PCR reaction was set up in 50 μL reaction volume: 5 μL 10X pfu turbox buffer; 1 μL 10μM dNTP; 2.5 μL 10 uM forward and reverse primer; 1 μL pfu turbo cx polymerase (Agilent); appropriate amount of DNA template and water added up to 50 μL.

The result showed that the density of the expected bands depended on the amount of DNA templates.

However, post-mortem DNA damage reactions, which fragment the DNA backbone into short pieces and generate hydrolytic and oxidative base derivatives, often limit the amount of DNA templates preserved [ 4- 6].

For STRs, genotyping panels designed for linkage scanning usually employ lower levels of multiplexing and use larger amounts of DNA template than do STR panels designed for forensic analysis, such as the AmpF lSTR® Identifiler™.

The gene dosage quotient was generated using peak height rather than peak area as an indicator of DNA template amount [ 39, 40].

Distinctive heteroduplex melting patterns were detected across the entire range of DNA template amounts, featuring an earlier melting of amplicon at the initial melting stage and becoming more stable at later stages compared with the wild-type.