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In this pilot study the alkaline comet assay offered information about the amount of DNA damage and the DNA repair status in PBL from individual patients and this seems useful in predicting the response to chemotherapy.
The apoptosis inhibiting activity of arsenite may have converted a greater amount of DNA damage to mutations without substantially affecting DNA repair.
Alternatively, different DNA repair responses could influence the amount of DNA damage and response to this single dose of CDDP.
The amount of DNA damage was higher in fpg modified Comet assay in comparison to alkaline Comet assay proving the oxidative stress induced DNA damage.
"The tub is your accumulated amount of DNA damage.
MIT researchers have developed an array of microscopic wells that can capture single cells and analyze the amount of DNA damage in each cell.
The amount of DNA damage correlated closely with the severity of malformations seen in individual larvae, and mutations in POLR1C led to far more DNA damage than mutations in POLR1D.
The amount of DNA migration under electric potential indicates the amount of DNA damage in the cell.
"What this means is that we've been underestimating the amount of DNA damage that people are getting from UV exposure," Brash says.
Comet assay or alkaline single-cell gel electrophoresis (SCGE) is a very simple, fast, and sensitive technique for assessing genotoxicity by quantifying the amount of DNA damage caused in individual cells.
Kopova et al. used immunofluorescence staining on the two fullerene films to determine the amount of DNA damage on genes that control the growth of human bone tissue (Fig. 2).
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