We also believe that the relatively small amount of cellular material in each well allows rapid quenching of treatments and preservation of the cell-interior by simple addition of fixing solution.
Although the amount of cellular material obtained by these procedures is relatively small, the information requested by the clinicians is rapidly growing, e.g., for NSCLC, immunohistochemistry and molecular pathology have become part of the standard care [12].
Measuring total RNA or genomic DNA gives a good idea of the amount of cellular material.
"Background blood or clot" and "amount of cellular material" are two important criteria in assessing the quality of smears [ 1].
However, the large amount of cellular material needed for standard biochemical assessment of signaling status has made it difficult to evaluate putative abnormalities completely using primary lymphocytes.
Although the viral transport media was not inhibitory to the PCR, the amount of cellular material may have decreased after this processing.
For Surepath samples, the tubes (enriched sample), not the vials, were used as the vials were found to have an inadequate amount of cellular material.
This scoring system consists of five objective parameters: (1) background blood or clot, (2) amount of cellular material, (3) degree of cellular degeneration, (4) degree of cellular trauma, and (5) retention of appropriate architecture (Table 1).
This false negative sample had the lowest amount of cellular material of all the BALs processed in this study which may reflect inadequate sampling of the lung segment at the time of bronchoscopy with BAL.
We investigated the requirement to deplete gDNA and rRNA in cultured viral samples after RNA extraction, since the amount of cellular material would be minimal in these supernatant preparations.
