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The mock control cells were transfected with equivalent amount of blank vector pCMV DNA or unrelated shRNA-generating DNA vector.
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Repeat this step until the desired amount of blank cards is completed.
In equivalent amounts of immunoprecipitated LOXL2 forms (Fig. 1B, bottom) the activity of ΔLOXL2 and H626/628Q mutants was similar to that of the blank vector control (Fig. 1B, upper), in concordance with the activity level previously reported in other catalytically inactive LOXL2 mutants (Lugassy et al., 2012; Herranz et al., 2012).
And it won't file unless you've filled in the proper blanks, there's a minimum amount of blanks you have to fill in.
Thus co-inoculation of effective type I IFNs with Blank vector at a total of 400μg/mouse resulted in abrogation of efficacy previously observed in this animal model.
Parallel control cultures of BMSCs (pMSCV-BMSCs) were transfected with blank vector.
For vector controls, the same amount of vector, pcDNA3.1, was injected into control oocytes instead of the isoforms.
The amount of vector DNA and the ratio of insert to vector DNA were examined.
To determine levels of break-induced minichromosome loss, background minichromosome loss at 48 h in blank vector assays was subtracted from break-induced minichromosome loss at 48 h in cells transformed with pREP81X-HO.
For vector-transfected controls, the same amount of an empty vector was transfected.
For live images of STIM1 translocation in MDCK cells, MDCK cells stably transfected with either murine PC1 or the blank vector in addition to the YFP-STIM1 construct.
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