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The IPN hydrogels were synthesized in two steps: (1) chitosan (CHT) crosslinked beads were obtained by reaction of chitosan with ethyleneglycol diglycidyl ether (EGDE); (2) absorption of a suitable amount of a solution of vinylpyrrolidone (VP) monomer from the porous CHT beads followed to VP polymerization and crosslinking inside the network.
The supernatant (800 μl) was mixed with the same amount of a solution that contained 0.4 M sucrose, 8 M urea, 5 mM EDTA and 5% (w/v) 2-mercaptoethanol.
Performing a dilution in chemistry usually means taking a small amount of a solution whose concentration you know, then adding a neutral liquid (like water) to make a new solution with a larger volume but a lower concentration.
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Briefly, an appropriate amount of a viscous solution (Pinpoint Solution, supplied in the kit) was applied over the area of cancer cells on the slide and air-dried to a thin film, together with the underlying cells.
Gel electrolytes were prepared by swelling a self-assembling ABA triblock copolymer with different amounts of a solution of 1 M lithium bis trifluoromethylsulfonyl)imide salt in γ-butyrolactone.
This study revealed that addition of an optimal amount of a CaCl2 solution successfully restored the coagulation ability of the anticoagulant-supplemented WB samples.
To use alcohol, apply a liberal amount of a 70% solution to all visible areas of fungus, and let it sit for ten minutes.
This was followed by titration with Fehling's solution, equal amounts of solution A (copper sulphate solution) and solution B (sodium potassium tartrate and sodium hydroxide solution) using methylene blue as indicator.
In that method, a partially polymerized solution of VDC, MMA and VTPCL in toluene, containing also an initiator, is dispersed at room temperature in a small amount of an aqueous solution of a surfactant.
A 500 mL amount of an aqueous solution of HAuCl4 ([Au3+] = 0.5 mM) was heated until heavy boiling.
pH adjustment of the inlet solutions was achieved by adding small amounts of a NaOH solution (0.1 mol/L) to the initial solution.
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