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The levels of methylated DNA of each sample were determined from the standard curve and combined with the amount input DNA to calculate the percentage methylation.
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The amount of total input DNA for each immunoprecipitation reaction was 140 μg.
Both the total protein amounts (inputs) and a negative control using the intracellular protein actin have been added to the figure (new Figure 3D) as requested.
The effect of diminishing amounts of input total RNA was also tested.
An increased amount of input DNA after digestion with McrBC was indicative of hypomethylation, whereas a decreased amount of input DNA was indicative of hypermethylation.
"There has been a huge amount of input both from experts and from the public.
They can be reproducibly genotyped from a low amount of input DNA.
The second was the amount of input from other employees that the leader considers while making a strategic decision.
The amount of input that is expected of you will be determined by the score assigned to you by The School's time-honored formula: Donations/I. Q
It looked as though the fairness doctrine offered a legal loophole that might allow me a large output for a small amount of input".
Due to the vast amount of input (water, fertilisers, fuel, etc).
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CEO of Professional Science Editing for Scientists @ prosciediting.com