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The water amount in the sample treated by saline is always higher for a given free volume hole size than in the case of deionized water.
Small degraded molecules appear accumulated in high amount in the sample solution and when measured; the concentration readings elevated more than the intact DNA molecules as portrayed by sample "a" in Table 1.
Drug loading was determined by HPLC as previously described [37] from the amount of drug in the supernatants and the total drug amount in the sample.
In parallel, a BSA range standard was used to correlate the OD with the total protein amount in the sample.
Correspondence between the cellulose amount in the sample and the pretreatment method that was applied can be found in Table 1.
Peak area was obtained for β-actin and APPL1 peptides, and the ratio of APPL1:β-actin was used to numerically evaluate protein amount in the sample.
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After the incubation, intact RNA amount in the samples was evaluated by gel electrophoresis analyses.
The DNA amount in the samples was quantified using a Pico 100 Picodrop Microliter Spectrophotometer (Picodrop Ltd., Hinxton, UK) according to the manufacturer's instructions.
In the following we will use differences in relative read abundances between metagenomes as an estimate of differences in relative DNA amounts in the sample.
We calculated the concentrations used in the analysis as the ratio of the metal amount in the PM10 sample to the air volume collected during the sampling.
Although viral amounts in the samples were not quantified, virus was detected in the cloacal and nasopharyngeal samples, usually only when the viral load in the kidney samples from the birds was high.
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