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For hippocampal neurons, the dissociated neurons from newborn mice were seeded for 10 days in regular Neurobasal A medium, which was then replaced with the corresponding D-AA medium for the designated amount of time before imaging.
For stability time course experiments, cells were incubated at 37 °C for the designated amount of time in the presence of serum-free RPMI 1640 before incubating with the fluorescent, complementary oligonucleotide.
For HeLa cells, cells were seeded on a coverslip in a Petri dish with 2 mL of regular medium for 20 h, which was then replaced with D-AA medium (or group I and group II D-AA media) for the designated amount of time.
Wait for the designated amount of time.
Pressure points can be sensitive and sore but try to massage each one that you've chosen for the designated amount of time or until the point begins to get numb to your touch.
The amount the designated charity will receive is preset; any contribution by the participant crowds out dollar-for-dollar giving by the proctor.
Iced water for the designated driver.
The amounts deducted are sent to the designated organizations.
Even in places where recreational marijuana has been legalized, possessing more than the designated amount puts you at risk for both misdemeanor and felony charges.
Fill the bottle with the designated amount of water.
Remove the pot from the heat after the designated amount of time has passed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com