Sentence examples for amount for library from inspiring English sources

A total of 2 ng purified cDNA products from each sample were used as the starting amount for library preparation.

PCR products were purified with QIAquick Gel extraction kit (Qiagen Inc. Valencia, CA, USA), then were mixed in equal amounts for library screening.

The amount of library required for template preparation was calculated using the template dilution factor calculation described in the protocol.

To create highly size-specific libraries, but preserve the maximum amount of DNA for library construction, the adapter-ligation product was size selected on a 2% agarose gel by collecting a series of ∼1-mm gel slices along the length of the gel lane, with mean insert sizes ranging from 209 to 638 bp (see Figure 1).

In pooling of multiple libraries for running on a single lane, equal amount of library mass was determined by Qubit reading and Bioanalyzer for the 5 RNA libraries.

For the "Replication" experiment, we processed two aliquots of DNA from individual A in parallel through the ligation step, using a different index for each aliquot, then pooled them in equal amounts for subsequent library preparation and sequencing.

As a consequence, more PCR cycles are required for larger insert libraries to obtain sufficient amounts of library DNA for NGS (Table  1).

Pooling of samples was required to obtain sufficient amounts of mRNA for library preparation.

Pooling of samples was necessary to achieve sufficient amounts of DNA for library preparation and has been previously used to reduce the effects of stochastic epigenetic variation [ 23].

The total amount paid to authors for library loans has dropped by 19.7% since 2005/06, when the pot was worth £7.6m.

Therefore we increased the number of semi-suppressive PCR cycles for those nanoCAGE library preparations to obtain the same DNA amounts for all libraries (Table  1).