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Four factors (variables), that are, sample solution pH, acceptor phase amount, extraction time and eluent concentration were optimized.
The parameters related to the μ-SPE procedure namely; pH, sorbent amount, extraction solvent type and volume, sonication and vortex time, sample volume and salt effect on the extraction efficiency were screened by applying Plackett Burmann design.
Briefly, in an L9 (3) orthogonal test, the solid liquid ratio, ethanol concentration, solvent amount, extraction times were determined.
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Fig. 1 The effect of the amount of extraction solvents on extracted Au III) signals.
Values for the amount and percentage of a constituent extracted by the subject were calculated for each pair (used and unused) of samples according to the following equations: Amount extracted = Quantity in unused pouch − Quantity in used pouch Percentage extraction = 100 × Amount extracted / Quantity in unused pouch.
For fecal antibody analysis, dry fecal pellets were weighed, and extracted in a proportionate amount of extraction buffer (1 ml PBS with 0.1 mg/ml trypsin inhibitor per 100 mg fecal pellet).
Figure 1a, b shows the median DNA levels measured per cell amount after extraction, their corresponding reference and the extrapolated limits of quantification for each DNA extraction method.
Typical FR peaks at 230 to 300 nm indicates its residual amount after extraction.
Besides, the amount of extraction of boric acid from ulexite depends on reaction conditions.
Orthogonal experiment design was utilized to optimize the amount of extraction solvent and disperser solvent, O-phenanthroline concentration and buffer pH.
As a result, a large amount of extraction wastewater is produced from paper pulp mills.
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