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Scientists at the Institute for Genomic Research in Rockville, Md., said they had found subtle differences among different samples of the Ames anthrax strain, increasing the possibility of identifying the laboratory that might have been the source of the microbes used in the attacks.
(PCA) revealed some similarities and differences among different samples from different regions of China.
It follows from these observations that pure sequence difference among different samples generated by SM-LATE-PCR must have been present in the initial population of molecules.
In order to accurately compare the relative abundance difference of P.acnes among different samples, human beta-actin was used as the internal reference.
The means and standard errors of qRT-PCR data among different samples were calculated, and the differences between them were determined by either t test or 1 way ANOVA, where p<0.05 is regarded as significant.
The chi-squared (χ2) test was used to analyze the difference in the expression levels among different samples.
Student's t test (P < 0.05) was used to determine the significant difference of relative expression of individual genes among different samples.
No significant difference was observed for the total dietary fiber content among different samples.
Similar TLC chromatograms were observed among different samples.
The method also reduces cross-contamination among different samples to a minimum.
The concentrations of five secondary metabolites exhibited a large variation among different samples.
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