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Among all mutants, Ala258Phe mutant displayed the highest activity of 7.59 U mg−1 and nearly 8-folds higher kcat/Km value towards xylose than wild-type BsGDH.
Among all mutants, strain CH7 carrying a nonsense mutation in marR (named marR109) and an insertion of IS5 in acrR, exhibited the highest organic solvent-tolerance levels.
By comparing the alteration in biological processes among all mutants, as shown in Table 3, distinct changes can be seen.
I-CXCL11 binding was robust among all mutants, except for the D112N mutant, which bound I-CXCL11 at detectable but significantly reduced levels compared with WT CXCR3.
The defects we observed among all mutants known to have growth cone or axonal outgrowth phenotypes were restricted to the distal trajectory of M1.
Among all mutants used in this study, R201K was the one with the lowest transport efficiency, i.e., nearly 9-fold lower than WT, confirming the crucial importance of this residue for Pi binding and translocation.
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However, among all the mutants tested, the areA mutant revealed the most severe growth defects.
Interestingly, among all the mutants of these dRecQ genes, only dRecQ4 mutants exhibit a homozygous lethal phenotype; mutants of the other dRecQ family members only show either fertility defects or various defects in DNA repair, including recombinational repair.
Among all the mutants and WT, the F146W mutant in the apo state shows the slowest hydration dynamics with only two decay components at 6.3 and 103 ps.
The differences observed in tropistic responses were not due to growth limitations since the growth rates among all the mutants tested were not significantly different from that of the WT.
D183N, in which one of the three salt bridges common to AQN and VPN was destroyed, was the most heat-labile among all the mutants tested in this study.
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