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Our prediction, based on the AML data, would be that the V391I RING finger domain mutation would affect the E3 activity.
Acute lymphoid leukaemia patient samples show overlapping, yet on average even lower levels of GATA2 than AML (data not shown) suggesting that the incompatibility of high GATA2 expression with leukaemia development may extend beyond AML.
AML: data acquisition, statistical analysis.
Here, we focus only on the AML data set.
In Table 7 we presented the summary of ranks for the AML data set.
Detecting the presence of batch effects in AML data was accomplished in two ways.
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Altered Twist1 level did not lead to changes in immunophenotypes of AML cells (data not shown).
We found that downregulation of CD82 by shRNAs decreased the level of CXCR4 in CD34+/CD38− AML cells (data not shown).
We also observed aberrant expression of genes encoding SNF2 family enzymes and CRC subunits in acute myeloid leukemia (AML) using data from the Cancer Genome Atlas (TCGA).
The MRC Medical Research Councill, UK) AML trial data show a consistent improvement in survival over time for younger, but not older, patients [ 6].
Currently however, except in some specific examples such as the BCR-ABL1 fusion in CML, and NPM1 or FLT3 mutations in de novo AML, molecular data are not associated with optimal clinical and therapeutic exploitation in the clinic.
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