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Biogenic amines were analyzed using high-performance liquid chromatography (HPLC) with electrochemical detection (Coulochem II, ESA) with a guard cell and a porous graphite "frit" flow cell both set at 660 mV.
For MS1-level analyses, all labeled amines were analyzed using an Orbitrap Exactive mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with a Thermo-Pal autosampler (Thermo Fisher Scientific, San Jose, CA) and an Accela 600 LC pump (Thermo Fisher Scientific, San Jose, CA).
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ΔΨm was analyzed using JC-1.
Extracts were vortexed and pelleted before the resulting supernatants were analyzed by LC-MS/MS using reversed phase chromatography with an amine-based ion pairing agent coupled to an electrospray mass spectrometer running in negative mode.
Finally, The effect of important process parameters such as CO2 partial pressure, CO2 loading of amine solution, flow rate of amine solution, concentration of amine solution and solution temperature on the CO2 removal efficiency were analyzed.
13 Where necessary the corresponding amines were prepared using literature procedures.
Amino acids and biogenic amines were quantitatively analyzed by reversed phase LC-MS/MS to obtain the chromatographic separation of isobaric (same MRM ion pairs) metabolites for individual quantification performed by external calibration and by use of internal standards.
Amino acids and biogenic amines were labelled by using their full name.
In this study, the effect of primary amine acetylation of the cationic, dendrimeric polymer, PAMAM, on siRNA delivery was analyzed.
To examine the use of pH as an electronic switch of structure, the peptide Ac-GPPXPPGY-NH2 (X = 4-aminophenylalanine) was analyzed by CD in both the ammonium (pH 3) and amine (pH 7) protonation states.
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