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In the core materials (5 to 10 cm in depth), the mineralogy was similar in both control and amended samples.
The efflorescent salts contained very soluble aluminium sulfates, together with alunite jarosite-group solid phalunite jarosite-groupsolidd cophasese-group solin phamended control.
The slope of the microbial growth curves representing microbial growth ability in C, CN, CP and CNP amended samples was taken as a measure of microbial nutrient limitation.
Copy numbers in the amended samples ranged from 2.275 to 3.775 × 10 (data not shown).
RNA from the amended samples contained an average of 70% the TRF numbers present in the un-amended controls.
In particular, 49.5% of the sequences from the amended samples showed homology to Zygomycete sp. AM-2008a, compared to 9% in the un-amended samples.
Finally, the amended sampling with Probability Proportionate to Size (PPS) is used to compensate for the errors or defects that may occur in the multi-stage sampling process [ 49].
The application of azoxystrobin significantly affected the numbers of TRFs (p = 0.04) (Table S2) with amended samples having, on average, 53% the number of TRFs of the unamended controls.
Our focus was on adaptation by amending sample size.
Finally, the method was applied to the determination of musks in vegetable and amended soil samples.
MDLs in the range of 0.01 0.8 ng/g and 0.01 1.1 ng/g were obtained for vegetables and amended soil samples, respectively.
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