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We did not succeed in obtaining transformants using competence induction methods developed for both B. subtilis [22] or an amended method for B. licheniformis [17] to induce natural competence.
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If there is a need to amend the method for selecting the Chief Executive for the terms subsequent to the year 2007, such amendments must be made with the endorsement of a two-thirds majority of all the members of the Legislative Council and the consent of the Chief Executive, and they shall be reported to the Standing Committee of the National People's Congress for approval.
The results show that our approach could effectively extend the modifying horizon of statistical models and has a better performance than the traditional linear method for amending short-term forecasts.
But the Human Rights Act lays down a fast track method for amending statutes judged incompatible with the European convention.
The court said the provision compelled speech from candidates in violation of the First Amendment; violated the constitutional "speech or debate" immunity enjoyed by members of Congress; added extra-constitutional qualifications for seeking office; and imposed an unconstitutional method for amending the Constitution.
The Laeken Declaration has established a fresh method for amending treaties.
If the Convention works well, this new method for amending European treaties will moreover become a permanent fixture.
It may include the following items: Basis of authority for the WUA, WUA Mission Statement, Basic roles and structure of the WUA, Legal status and basis of authority, Area of jurisdiction, Criteria for membership (including for non-agricultural water users), Basic rights, powers and obligations of the WUA and its members, Structure of leadership, Method for amending Articles of Association.
The two methods are amended to account for these factors.
The Methods have be amended as follows: "For GFP-Trap® affinity purification, cells were lysed in the manufacturer's recommended buffers (Chromotek, Germany) and incubated with GFP-Trap® A beads according to manufacturer's instructions.
We amend the method to calculate the ETX of a specific link.
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