To develop an FMO amenable to crystallization, we introduced mutations into rabbit FMO2 (rF2) to increase solubility, decrease aggregation, and simplify isolation.
DOI: http://dx.doi.org/10.7554/eLife.04745.004 Having established the presence, in the 40k strain, of deleterious mutations potentially amenable to buffering, we introduced plasmids carrying a specific E. coli DBRH gene (either csdA, rhlB, or srmB) into each of the three genetic backgrounds (ancestor, 20k, and 40k).
Drug experts like Westley Clark, director of the Centre for Substance Abuse Treatment in Rockville, Maryland, are hoping to introduce more amenable means of distributing newer, potentially more effective drugs for heroin treatment (such as buprenorphine, now in clinical trials in America) to these addicts.
Here, we have presented a proof-of-concept demonstration for the generation and application of oligo pools that specifically target thousands of unique chromosomal loci across a cell population to introduce promoters amenable for synthetic regulation.
To describe the situation, it is convenient to introduce the class (mathcal{AG }) of amenable groups, and the subclass (mathcal{EG }) of elementary amenable groups, which is easier to understand (Appendix D is a reminder on these classes, and on growth).
Therefore, it is necessary to introduce some cost effective pretreatment processes to make the biomass polysaccharides easily amenable to enzymatic attack to release mixed fermentable sugars.
The structure of CPT is easily amenable to chemical modifications aimed at introducing substituents to reinforce target inhibition and/or to improve water-solubility.
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Therefore, our data are not amenable to survival analysis, and thus bias introduced by differential follow-up between groups is possible.
